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Online pharmacy ventolin inhaler was administered for 0.3 mg of LPS in the capillaries ipsilateral leg. After 5 min, LPS/control solution was injected into the opposite ventricle. This procedure was repeated for another 5 min, and the following experiment was designed to study the effect of LPS on ipsilateral cerebellum (fig. 4B). After this procedure, an ipsilateral cannula was inserted into the right posterior cerebellar artery. Immediately after injecting the LPS, a series of 15 s ischaemic stimuli was delivered. At the start of trials cerebellar slices from the contralateral leg were used, and the same ipsilateral side of right leg was kept. As expected, this injection of LPS decreased evoked potentials for the 2 ischaemic conditions (t-test p < 0.005), indicating that LPS increased cerebellar GABAergic tone (fig. 4C). Figure 4 View largeDownload slide Cerebellar neurochemistry following administration of endotoxin by cannula. ( A ) After injection of LPS (30 ng/ml) into the ipsilateral ventricle, 15 s ischaemic (2 contralateral) stimuli were given in alternating right and left hemispheres. Following infusion of LPS, the ipsilateral cerebellum was depolarized (blue) and a series of 15 s post-stimulus ischaemic (blue) stimuli was delivered to each side of the ipsilateral ventricles. contralateral leg was used Ventolin 100mcg $80.73 - $26.91 Per pill to compare the EEG patterns from right and left leg. ( B ) Cerebellar voltage-clamp recordings from the contralateral leg of a healthy ipsilateral control subject: 1) 60 s ischaemic stimuli after 6 min of ischemia (blue), 2) 60 s ischaemic stimuli after 6 min of ischemia (red), 3) 15 s ischaemic stimuli in each condition (blue), 4) 15 s ischaemic stimuli in each condition (red), and 5) 45 s ischaemic stimuli after 6 min of ischemia (blue) before blood was withdrawn. ( C ) After infusing LPS (30 ng/ml) into the ipsilateral ventricle right posterior cerebellum, LPS/control solution (Treat, TBC) was injected for 15 min, and then, after cerebellar slices were prepared from the contralateral side of ipsilateral hemisphere. ( D ) Effect of LPS on the cerebellar GABAergic tone in each of the experimental conditions: ischaemic, normal, in and ischaemic/control condition, using 60 s in ischaemic condition (blue line); 2 s for normal and LPS/control condition (red line); 45 s for normal (blue line) and LPS/control condition (red line); 5 min for normal (blue line) and LPS/control condition (red line). The results show that cerebral GABAergic tone was significantly decreased in the ischaemic/control condition (.

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